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Superglue from bacteria: unbreakable bridges for protein nanotechnology
#MMPMID25168413
Veggiani G
; Zakeri B
; Howarth M
Trends Biotechnol
2014[Oct]; 32
(10
): 506-12
PMID25168413
show ga
Biotechnology is often limited by weak interactions. We suggest that an ideal
interaction between proteins would be covalent, specific, require addition of
only a peptide tag to the protein of interest, and form under a wide range of
conditions. Here we summarize peptide tags that are able to form spontaneous
amide bonds, based on harnessing reactions of adhesion proteins from the
bacterium Streptococcus pyogenes. These include the irreversible peptide-protein
interaction of SpyTag with SpyCatcher, as well as irreversible peptide-peptide
interactions via SpyLigase. We describe existing applications, including
polymerization to enhance cancer cell capture, assembly of living biomaterial,
access to diverse protein shapes, and improved enzyme resilience. We also
indicate future opportunities for resisting biological force and extending the
scope of protein nanotechnology.
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