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10.1364/BOE.9.001613 http://scihub22266oqcxt.onion/10.1364/BOE.9.001613 C5905910!5905910 !29675306     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29675306 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biomed+Opt+Express 2018 ; 9 (4 ): 1613-1629 Nephropedia Template TP {{tp|p=29675306 |t=2018. Super-resolution fluorescence microscopy by stepwise optical saturation |pdf=|usr=}}{{29675306 }} gab.com Text Super-resolution fluorescence microscopy by stepwise optical saturation JO: Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=29675306 #FOAvip Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy is not feasible in many applications. In this paper, we propose and demonstrate a saturation-based super-resolution fluorescence microscopy technique that can be easily implemented and requires neither additional hardware nor complex post-processing. The method is based on the principle of stepwise optical saturation (SOS), where M steps of raw fluorescence images are linearly combined to generate an image with a [Formula: see text]-fold increase in resolution compared with conventional diffraction-limited images. For example, linearly combining (scaling and subtracting) two images obtained at regular powers extends the resolution by a factor of 1.4 beyond the diffraction limit. The resolution improvement in SOS microscopy is theoretically infinite but practically is limited by the signal-to-noise ratio. We perform simulations and experimentally demonstrate super-resolution microscopy with both one-photon (confocal) and multiphoton excitation fluorescence. We show that with the multiphoton modality, the SOS microscopy can provide super-resolution imaging deep in scattering samples. Twit Text FOAVip Super-resolution fluorescence microscopy by stepwise optical saturation ????Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=29675306 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29675306 #FOAvip English Wikipedia <ref>{{Cite pmid|29675306 }}</ref> Super-resolution fluorescence microscopy by stepwise optical saturation #MMPMID29675306 Zhang Y ; Nallathamby PD ; Vigil GD ; Khan AA ; Mason DE ; Boerckel JD ; Roeder RK ; Howard SS Biomed Opt Express 2018[Apr]; 9 (4 ): 1613-1629 PMID29675306 show ga Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy is not feasible in many applications. In this paper, we propose and demonstrate a saturation-based super-resolution fluorescence microscopy technique that can be easily implemented and requires neither additional hardware nor complex post-processing. The method is based on the principle of stepwise optical saturation (SOS), where M steps of raw fluorescence images are linearly combined to generate an image with a [Formula: see text]-fold increase in resolution compared with conventional diffraction-limited images. For example, linearly combining (scaling and subtracting) two images obtained at regular powers extends the resolution by a factor of 1.4 beyond the diffraction limit. The resolution improvement in SOS microscopy is theoretically infinite but practically is limited by the signal-to-noise ratio. We perform simulations and experimentally demonstrate super-resolution microscopy with both one-photon (confocal) and multiphoton excitation fluorescence. We show that with the multiphoton modality, the SOS microscopy can provide super-resolution imaging deep in scattering samples. äSuper-resolution fluorescence microscopy by stepwise optical saturatio(epmc).pdf DeepDyve Pubget Overpricing