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2016 ; 11
(10
): e0165194
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Super-Resolution Real Imaging in Microsphere-Assisted Microscopy
#MMPMID27768774
Lai HS
; Wang F
; Li Y
; Jia B
; Liu L
; Li WJ
PLoS One
2016[]; 11
(10
): e0165194
PMID27768774
show ga
Microsphere-assisted microscopy has received a lot of attention recently due to
its simplicity and its capability to surpass the diffraction limit. However, to
date, sub-diffraction-limit features have only been observed in virtual images
formed through the microspheres. We show that it is possible to form real,
super-resolution images using high-refractive index microspheres. Also, we report
on how changes to a microsphere's refractive index and size affect image
formation and planes. The relationship between the focus position and the
additional magnification factor is also investigated using experimental and
theoretical methods. We demonstrate that such a real imaging mode, combined with
the use of larger microspheres, can enlarge sub-diffraction-limit features up to
10 times that of wide-field microscopy's magnification with a field-of-view
diameter of up to 9 ?m.