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2014 ; 2014
(7
): 737-40
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Subcapsular transplantation of tissue in the kidney
#MMPMID24987138
Shultz LD
; Goodwin N
; Ishikawa F
; Hosur V
; Lyons BL
; Greiner DL
Cold Spring Harb Protoc
2014[Jul]; 2014
(7
): 737-40
PMID24987138
show ga
Multiple sites can be used for the engraftment of primary human cells and tissues
into murine hosts. For example, leukemias are usually best engrafted
intravenously, but they can also be engrafted directly into the bone marrow
cavity. Some solid tumors such as colon tumors grow successfully following
subcutaneous engraftment, sometimes requiring provision of a Matrigel artificial
basement membrane. In certain cases (e.g., human bladder cancer and ductal breast
carcinoma), the use of the autochthonous site (bladder and mammary duct,
respectively) is often most efficient, whereas the tumors can grow poorly when
transplanted subcutaneously or heterochthonously. Here, we present a protocol for
the surgical implantation of tissues under the kidney capsule. The kidney is
especially suited for the transplantation of normal as well as malignant cells
and tissues. It is very accessible, and transplanted tissues are well contained
under the renal capsule in a highly vascularized site. Furthermore, the
retroperitoneal location of the kidney, together with its separation from other
organs, is advantageous both for imaging and biopsy.
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