Study of apoptosis in human liver cancers
#MMPMID11925601
Shan CM
; Li J
World J Gastroenterol
2002[Apr]; 8
(2
): 247-52
PMID11925601
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AIM: To investigate the action of apoptosis in occurrence of liver carcinomas in
vivo and the biological effect of Solanum lyratum Thumb on BEL-7404 cell line
inducing apoptosis in vivo. METHODS: The apoptosis in the liver carcinoma was
detected with terminal deoxynucl neotidyl transferase mediated dUTP nick end
labelling (TUNEL); the cancer cells cultured in DMED medium were treated with
extract of Solanum lyratum Thumb and observed under microscope, and their DNA was
assayed by gel electrophoresis. RESULTS: In vivo apoptotic cells in the cancer
adjacent tissues inceased; in vivo treatment of liver cancers with extract of
Solanum lyratum Thumb could induce the cells to manifest a typical apoptotic
morphology. Their DNA was fractured and a characteristic ladder pattern could be
found using electrophoresis. CONCLUSION: In vivo the apoptosis of carcinomas was
lower; maybe the cells divided quickly and then the cancers occurred. In the
cancer adjacent tissues,the apoptosis pricked up, and in vivo Solanum lyratum
Thumb could induce the apoptosis of BEL-7404 cells.
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