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10.1038/s41598-017-02438-0 http://scihub22266oqcxt.onion/10.1038/s41598-017-02438-0 C5446423!5446423 !28550293     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28550293 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Sci+Rep 2017 ; 7 (1 ): 2448 Nephropedia Template TP {{tp|p=28550293 |t=2017. Stochastic sensing of Angiotensin II with lysenin channels |pdf=|usr=}}{{28550293 }} gab.com Text Stochastic sensing of Angiotensin II with lysenin channels JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28550293 #FOAvip The ability of pore-forming proteins to interact with various analytes has found vast applicability in single molecule sensing and characterization. In spite of their abundance in organisms from all kingdoms of life, only a few pore-forming proteins have been successfully reconstituted in artificial membrane systems for sensing purposes. Lysenin, a pore-forming toxin extracted from the earthworm E. fetida, inserts large conductance nanopores in lipid membranes containing sphingomyelin. Here we show that single lysenin channels may function as stochastic nanosensors by allowing the short cationic peptide angiotensin II to be electrophoretically driven through the conducting pathway. Long-term translocation experiments performed using large populations of lysenin channels allowed unequivocal identification of the unmodified analyte by Liquid Chromatography-Mass Spectrometry. However, application of reverse voltages or irreversible blockage of the macroscopic conductance of lysenin channels by chitosan addition prevented analyte translocation. This investigation demonstrates that lysenin channels have the potential to function as nano-sensing devices capable of single peptide molecule identification and characterization, which may be further extended to other macromolecular analytes. Twit Text FOAVip Stochastic sensing of Angiotensin II with lysenin channels ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28550293 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28550293 #FOAvip English Wikipedia <ref>{{Cite pmid|28550293 }}</ref> Stochastic sensing of Angiotensin II with lysenin channels #MMPMID28550293 Shrestha N ; Bryant SL ; Thomas C ; Richtsmeier D ; Pu X ; Tinker J ; Fologea D Sci Rep 2017[May]; 7 (1 ): 2448 PMID28550293 show ga The ability of pore-forming proteins to interact with various analytes has found vast applicability in single molecule sensing and characterization. In spite of their abundance in organisms from all kingdoms of life, only a few pore-forming proteins have been successfully reconstituted in artificial membrane systems for sensing purposes. Lysenin, a pore-forming toxin extracted from the earthworm E. fetida, inserts large conductance nanopores in lipid membranes containing sphingomyelin. Here we show that single lysenin channels may function as stochastic nanosensors by allowing the short cationic peptide angiotensin II to be electrophoretically driven through the conducting pathway. Long-term translocation experiments performed using large populations of lysenin channels allowed unequivocal identification of the unmodified analyte by Liquid Chromatography-Mass Spectrometry. However, application of reverse voltages or irreversible blockage of the macroscopic conductance of lysenin channels by chitosan addition prevented analyte translocation. This investigation demonstrates that lysenin channels have the potential to function as nano-sensing devices capable of single peptide molecule identification and characterization, which may be further extended to other macromolecular analytes. |Angiotensin II/*chemistry/metabolism [MESH] |Animals [MESH] |Biological Transport/drug effects [MESH] |Biosensing Techniques/methods [MESH] |Chitosan/pharmacology [MESH] |Ion Channel Gating/drug effects [MESH] |Lipid Bilayers/*chemistry/metabolism [MESH] |Oligochaeta/*chemistry [MESH] |Sphingomyelins/chemistry/metabolism [MESH]Stochastic sensing of Angiotensin II with lysenin channels (epmc).pdf DeepDyve Pubget Overpricing