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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nat+Photonics
2016 ; 10
(8
): 534-540
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Stain-free histopathology by programmable supercontinuum pulses
#MMPMID27668009
Tu H
; Liu Y
; Turchinovich D
; Marjanovic M
; Lyngsø J
; Lægsgaard J
; Chaney EJ
; Zhao Y
; You S
; Wilson WL
; Xu B
; Dantus M
; Boppart SA
Nat Photonics
2016[Aug]; 10
(8
): 534-540
PMID27668009
show ga
The preparation, staining, visualization, and interpretation of histological
images of tissue is well-accepted as the gold standard process for the diagnosis
of disease. These methods were developed historically, and are used ubiquitously
in pathology, despite being highly time and labor intensive. Here we introduce a
unique optical imaging platform and methodology for label-free multimodal
multiphoton microscopy that uses a novel photonic crystal fiber source to
generate tailored chemical contrast based on programmable supercontinuum pulses.
We demonstrate collection of optical signatures of the tumor microenvironment,
including evidence of mesoscopic biological organization, tumor cell migration,
and (lymph-)angiogenesis collected directly from fresh ex vivo mammary tissue.
Acquisition of these optical signatures and other cellular or extracellular
features, which are largely absent from histologically processed and stained
tissue, combined with an adaptable platform for optical alignment-free
programmable-contrast imaging, offers the potential to translate stain-free
molecular histopathology into routine clinical use.