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2014 ; 5
(8
): e1369
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Stabilization of apoptotic cells: generation of zombie cells
#MMPMID25118929
Oropesa-Ávila M
; Andrade-Talavera Y
; Garrido-Maraver J
; Cordero MD
; de la Mata M
; Cotán D
; Paz MV
; Pavón AD
; Alcocer-Gómez E
; de Lavera I
; Lema R
; Zaderenko AP
; Rodríguez-Moreno A
; Sánchez-Alcázar JA
Cell Death Dis
2014[Aug]; 5
(8
): e1369
PMID25118929
show ga
Apoptosis is characterized by degradation of cell components but plasma membrane
remains intact. Apoptotic microtubule network (AMN) is organized during apoptosis
forming a cortical structure beneath plasma membrane that maintains plasma
membrane integrity. Apoptotic cells are also characterized by high reactive
oxygen species (ROS) production that can be potentially harmful for the cell. The
aim of this study was to develop a method that allows stabilizing apoptotic cells
for diagnostic and therapeutic applications. By using a cocktail composed of
taxol (a microtubule stabilizer), Zn(2+) (a caspase inhibitor) and coenzyme Q10
(a lipid antioxidant), we were able to stabilize H460 apoptotic cells in cell
cultures for at least 72?h, preventing secondary necrosis. Stabilized apoptotic
cells maintain many apoptotic cell characteristics such as the presence of
apoptotic microtubules, plasma membrane integrity, low intracellular calcium
levels and mitochondrial polarization. Apoptotic cell stabilization may open new
avenues in apoptosis detection and therapy.
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