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2012 ; 35
(5-6
): 339-61
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Spectral imaging in preclinical research and clinical pathology
#MMPMID22475632
Levenson R
; Beechem J
; McNamara G
Anal Cell Pathol (Amst)
2012[]; 35
(5-6
): 339-61
PMID22475632
show ga
Spectral imaging methods are attracting increased interest from researchers and
practitioners in basic science, pre-clinical and clinical arenas. A combination
of better labeling reagents and better optics creates opportunities to detect and
measure multiple parameters at the molecular and cellular level. These tools can
provide valuable insights into the basic mechanisms of life, and yield diagnostic
and prognostic information for clinical applications. There are many
multispectral technologies available, each with its own advantages and
limitations. This chapter will present an overview of the rationale for spectral
imaging, and discuss the hardware, software and sample labeling strategies that
can optimize its usefulness in clinical settings.