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10.1007/978-1-4939-2877-4_5 http://scihub22266oqcxt.onion/10.1007/978-1-4939-2877-4_5 C4887086!4887086 !26404144     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26404144 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2015 ; 1334 (ä): 85-99 Nephropedia Template TP {{tp|p=26404144 |t=2015. Southwestern Blotting Assay |pdf=|usr=}}{{26404144 }} gab.com Text Southwestern Blotting Assay JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26404144 #FOAvip Southwestern blotting is a technique used to study DNA-protein interactions. This method detects specific DNA-binding proteins by incubating radiolabeled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electrophoresis, such as a gel shift (EMSA), can precede isoelectric focusing and SDS-PAGE to further improve separation. Combined with other techniques, such as mass spectrometry, the DNA-binding protein can be identified. Twit Text FOAVip Southwestern Blotting Assay ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=26404144 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26404144 #FOAvip English Wikipedia <ref>{{Cite pmid|26404144 }}</ref> Southwestern Blotting Assay #MMPMID26404144 Jia Y ; Nagore L ; Jarrett H Methods Mol Biol 2015[]; 1334 (ä): 85-99 PMID26404144 show ga Southwestern blotting is a technique used to study DNA-protein interactions. This method detects specific DNA-binding proteins by incubating radiolabeled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electrophoresis, such as a gel shift (EMSA), can precede isoelectric focusing and SDS-PAGE to further improve separation. Combined with other techniques, such as mass spectrometry, the DNA-binding protein can be identified. |Blotting, Southwestern/*methods [MESH] |DNA-Binding Proteins/*chemistry/genetics [MESH] |DNA/*chemistry/genetics [MESH] |Electrophoresis, Gel, Two-Dimensional [MESH] |Electrophoretic Mobility Shift Assay/*methods [MESH] |Mass Spectrometry [MESH]Southwestern Blotting Assay (epmc).pdf DeepDyve Pubget Overpricing