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2008 ; 321
(5891
): 960-4
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Small CRISPR RNAs guide antiviral defense in prokaryotes
#MMPMID18703739
Brouns SJ
; Jore MM
; Lundgren M
; Westra ER
; Slijkhuis RJ
; Snijders AP
; Dickman MJ
; Makarova KS
; Koonin EV
; van der Oost J
Science
2008[Aug]; 321
(5891
): 960-4
PMID18703739
show ga
Prokaryotes acquire virus resistance by integrating short fragments of viral
nucleic acid into clusters of regularly interspaced short palindromic repeats
(CRISPRs). Here we show how virus-derived sequences contained in CRISPRs are used
by CRISPR-associated (Cas) proteins from the host to mediate an antiviral
response that counteracts infection. After transcription of the CRISPR, a complex
of Cas proteins termed Cascade cleaves a CRISPR RNA precursor in each repeat and
retains the cleavage products containing the virus-derived sequence. Assisted by
the helicase Cas3, these mature CRISPR RNAs then serve as small guide RNAs that
enable Cascade to interfere with virus proliferation. Our results demonstrate
that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of
Cascade is a mechanistic requirement for antiviral defense.
|*Bacteriophage lambda/genetics/growth & development
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