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10.1016/j.jsb.2015.09.010 http://scihub22266oqcxt.onion/10.1016/j.jsb.2015.09.010 C4633364!4633364 !26409249     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26409249 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Struct+Biol 2015 ; 192 (2 ): 151-8 Nephropedia Template TP {{tp|p=26409249 |t=2015. Site-specific labeling of proteins for electron microscopy |pdf=|usr=}}{{26409249 }} gab.com Text Site-specific labeling of proteins for electron microscopy JO: J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=26409249 #FOAvip Electron microscopy is commonly employed to determine the subunit organization of large macromolecular assemblies. However, the field lacks a robust molecular labeling methodology for unambiguous identification of constituent subunits. We present a strategy that exploits the unique properties of an unnatural amino acid in order to enable site-specific attachment of a single, readily identifiable protein label at any solvent-exposed position on the macromolecular surface. Using this method, we show clear labeling of a subunit within the 26S proteasome lid subcomplex that has not been amenable to labeling by traditional approaches. Twit Text FOAVip Site-specific labeling of proteins for electron microscopy ????J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=26409249 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26409249 #FOAvip English Wikipedia <ref>{{Cite pmid|26409249 }}</ref> Site-specific labeling of proteins for electron microscopy #MMPMID26409249 Dambacher CM ; Lander GC J Struct Biol 2015[Nov]; 192 (2 ): 151-8 PMID26409249 show ga Electron microscopy is commonly employed to determine the subunit organization of large macromolecular assemblies. However, the field lacks a robust molecular labeling methodology for unambiguous identification of constituent subunits. We present a strategy that exploits the unique properties of an unnatural amino acid in order to enable site-specific attachment of a single, readily identifiable protein label at any solvent-exposed position on the macromolecular surface. Using this method, we show clear labeling of a subunit within the 26S proteasome lid subcomplex that has not been amenable to labeling by traditional approaches. |Amino Acids/chemistry [MESH] |Click Chemistry/methods [MESH] |Escherichia coli/genetics/metabolism [MESH] |Imaging, Three-Dimensional/methods [MESH] |Macromolecular Substances/analysis [MESH] |Microscopy, Electron/*methods [MESH] |Models, Molecular [MESH] |Proteasome Endopeptidase Complex/*analysis/*chemistry [MESH] |Proteins/*analysis [MESH]Site-specific labeling of proteins for electron microscopy (epmc).pdf DeepDyve Pubget Overpricing