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2015 ; 10
(4
): e0123216
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Sinonasal microbiome sampling: a comparison of techniques
#MMPMID25876035
Bassiouni A
; Cleland EJ
; Psaltis AJ
; Vreugde S
; Wormald PJ
PLoS One
2015[]; 10
(4
): e0123216
PMID25876035
show ga
BACKGROUND: The role of the sino-nasal microbiome in CRS remains unclear. We
hypothesized that the bacteria within mucosal-associated biofilms may be
different from the more superficial-lying, free-floating bacteria in the sinuses
and that this may impact on the microbiome results obtained. This study
investigates whether there is a significant difference in the microbiota of a
sinonasal mucosal tissue sample versus a swab sample. METHODS: Cross-sectional
study with paired design. Mucosal biopsy and swab samples were obtained
intra-operatively from the ethmoid sinuses of 6 patients with CRS. Extracted DNA
was sequenced on a Roche-454 sequencer using 16S-rRNA gene targeted primers. Data
were analyzed using QIIME 1.8 software package. RESULTS: At a maximum subsampling
depth of 1,100 reads, the mean observed species richness was 33.3 species (30.6
for swab, versus 36 for mucosa; p > 0.05). There was no significant difference in
phylogenetic and non-phylogenetic alpha diversity metrics (Faith's PD_Whole_Tree
and Shannon's index) between the two sampling methods (p > 0.05). The type of
sample also had no significant effect on phylogenetic and non-phylogenetic beta
diversity metrics (Unifrac and Bray-Curtis; p > 0.05). CONCLUSION: We observed no
significant difference between the microbiota of mucosal tissue and swab samples.
This suggests that less invasive swab samples are representative of the sinonasal
mucosa microbiome and can be used for future sinonasal microbiome studies.
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