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2016 ; 65
(1
): 9-22
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Single-particle cryo-electron microscopy of macromolecular complexes
#MMPMID26611544
Skiniotis G
; Southworth DR
Microscopy (Oxf)
2016[Feb]; 65
(1
): 9-22
PMID26611544
show ga
Recent technological breakthroughs in image acquisition have enabled
single-particle cryo-electron microscopy (cryo-EM) to achieve near-atomic
resolution structural information for biological complexes. The improvements in
image quality coupled with powerful computational methods for sorting distinct
particle populations now also allow the determination of compositional and
conformational ensembles, thereby providing key insights into macromolecular
function. However, the inherent instability and dynamic nature of biological
assemblies remain a tremendous challenge that often requires tailored approaches
for successful implementation of the methodology. Here, we briefly describe the
fundamentals of single-particle cryo-EM with an emphasis on covering the breadth
of techniques and approaches, including low- and high-resolution methods, aiming
to illustrate specific steps that are crucial for obtaining structural
information by this method.
free
free
free
