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10.1016/j.ab.2017.03.015 http://scihub22266oqcxt.onion/10.1016/j.ab.2017.03.015 C5814351!5814351 !28322800     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28322800 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Anal+Biochem 2018 ; 552 (ä): 45-49 Nephropedia Template TP {{tp|p=28322800 |t=2018. Single molecule mtDNA fiber FISH for analyzing numtogenesis |pdf=|usr=}}{{28322800 }} gab.com Text Single molecule mtDNA fiber FISH for analyzing numtogenesis JO: Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=28322800 #FOAvip Somatic human cells contain thousands of copies of mitochondrial DNA (mtDNA). In eukaryotes, natural transfer of mtDNA into the nucleus generates nuclear mitochondrial DNA (NUMT) copies. We name this phenomenon as "numtogenesis". Numtogenesis is a well-established evolutionary process reported in various sequenced eukaryotic genomes. We have established a molecular tool to rapidly detect and analyze NUMT insertions in whole genomes. To date, NUMT analyses depend on deep genome sequencing combined with comprehensive computational analyses of the whole genome. This is time consuming, cumbersome and cost prohibitive. Further, most laboratories cannot accomplish such analyses due to limited skills. We report the development of single-molecule mtFIBER FISH (fluorescence in situ hybridization) to study numtogenesis. The development of mtFIBER FISH should aid in establishing a role for numtogenesis in cancers and other human diseases. This novel technique should help distinguish and monitor cancer stages and progression, aid in elucidation of basic mechanisms underlying tumorigenesis and facilitate analyses of processes related to early detection of cancer, screening and/or cancer risk assessment. Twit Text FOAVip Single molecule mtDNA fiber FISH for analyzing numtogenesis ????Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=28322800 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28322800 #FOAvip English Wikipedia <ref>{{Cite pmid|28322800 }}</ref> Single molecule mtDNA fiber FISH for analyzing numtogenesis #MMPMID28322800 Koo DH ; Singh B ; Jiang J ; Friebe B ; Gill BS ; Chastain PD ; Manne U ; Tiwari HK ; Singh KK Anal Biochem 2018[Jul]; 552 (ä): 45-49 PMID28322800 show ga Somatic human cells contain thousands of copies of mitochondrial DNA (mtDNA). In eukaryotes, natural transfer of mtDNA into the nucleus generates nuclear mitochondrial DNA (NUMT) copies. We name this phenomenon as "numtogenesis". Numtogenesis is a well-established evolutionary process reported in various sequenced eukaryotic genomes. We have established a molecular tool to rapidly detect and analyze NUMT insertions in whole genomes. To date, NUMT analyses depend on deep genome sequencing combined with comprehensive computational analyses of the whole genome. This is time consuming, cumbersome and cost prohibitive. Further, most laboratories cannot accomplish such analyses due to limited skills. We report the development of single-molecule mtFIBER FISH (fluorescence in situ hybridization) to study numtogenesis. The development of mtFIBER FISH should aid in establishing a role for numtogenesis in cancers and other human diseases. This novel technique should help distinguish and monitor cancer stages and progression, aid in elucidation of basic mechanisms underlying tumorigenesis and facilitate analyses of processes related to early detection of cancer, screening and/or cancer risk assessment. |Biological Transport [MESH] |Cell Line, Tumor [MESH] |Cell Nucleus/genetics/*metabolism [MESH] |DNA, Mitochondrial/*metabolism [MESH] |Humans [MESH]Single molecule mtDNA fiber FISH for analyzing numtogenesis (epmc).pdf DeepDyve Pubget Overpricing