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2016 ; 4
(ä): 116
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Single Cell Isolation and Analysis
#MMPMID27826548
Hu P
; Zhang W
; Xin H
; Deng G
Front Cell Dev Biol
2016[]; 4
(ä): 116
PMID27826548
show ga
Individual cell heterogeneity within a population can be critical to its peculiar
function and fate. Subpopulations studies with mixed mutants and wild types may
not be as informative regarding which cell responds to which drugs or clinical
treatments. Cell to cell differences in RNA transcripts and protein expression
can be key to answering questions in cancer, neurobiology, stem cell biology,
immunology, and developmental biology. Conventional cell-based assays mainly
analyze the average responses from a population of cells, without regarding
individual cell phenotypes. To better understand the variations from cell to
cell, scientists need to use single cell analyses to provide more detailed
information for therapeutic decision making in precision medicine. In this
review, we focus on the recent developments in single cell isolation and
analysis, which include technologies, analyses and main applications. Here, we
summarize the historical background, limitations, applications, and potential of
single cell isolation technologies.