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10.1083/jcb.201510064 http://scihub22266oqcxt.onion/10.1083/jcb.201510064 C4810306!4810306 !27022090     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27022090 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27022090 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Biol 2016 ; 212 (7 ): 777-87 Nephropedia Template TP {{tp|p=27022090 |t=2016. Shape-motion relationships of centering microtubule asters |pdf=|usr=}}{{27022090 }} gab.com Text Shape-motion relationships of centering microtubule asters JO: J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=27022090 #FOAvip Although mechanisms that contribute to microtubule (MT) aster positioning have been extensively studied, still little is known on how asters move inside cells to faithfully target a cellular location. Here, we study sperm aster centration in sea urchin eggs, as a stereotypical large-scale aster movement with extreme constraints on centering speed and precision. By tracking three-dimensional aster centration dynamics in eggs with manipulated shapes, we show that aster geometry resulting from MT growth and interaction with cell boundaries dictates aster instantaneous directionality, yielding cell shape-dependent centering trajectories. Aster laser surgery and modeling suggest that dynein-dependent MT cytoplasmic pulling forces that scale to MT length function to convert aster geometry into directionality. In contrast, aster speed remains largely independent of aster size, shape, or absolute dynein activity, which suggests it may be predominantly determined by aster growth rate rather than MT force amplitude. These studies begin to define the geometrical principles that control aster movements. Twit Text FOAVip Shape-motion relationships of centering microtubule asters ????J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=27022090 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27022090 #FOAvip English Wikipedia <ref>{{Cite pmid|27022090 }}</ref> Shape-motion relationships of centering microtubule asters #MMPMID27022090 Tanimoto H ; Kimura A ; Minc N J Cell Biol 2016[Mar]; 212 (7 ): 777-87 PMID27022090 show ga Although mechanisms that contribute to microtubule (MT) aster positioning have been extensively studied, still little is known on how asters move inside cells to faithfully target a cellular location. Here, we study sperm aster centration in sea urchin eggs, as a stereotypical large-scale aster movement with extreme constraints on centering speed and precision. By tracking three-dimensional aster centration dynamics in eggs with manipulated shapes, we show that aster geometry resulting from MT growth and interaction with cell boundaries dictates aster instantaneous directionality, yielding cell shape-dependent centering trajectories. Aster laser surgery and modeling suggest that dynein-dependent MT cytoplasmic pulling forces that scale to MT length function to convert aster geometry into directionality. In contrast, aster speed remains largely independent of aster size, shape, or absolute dynein activity, which suggests it may be predominantly determined by aster growth rate rather than MT force amplitude. These studies begin to define the geometrical principles that control aster movements. |*Cell Movement [MESH] |*Cell Shape [MESH] |*Mechanotransduction, Cellular [MESH] |*Sperm-Ovum Interactions [MESH] |Animals [MESH] |Dyneins/metabolism [MESH] |Female [MESH] |Imaging, Three-Dimensional [MESH] |Laser Therapy [MESH] |Male [MESH] |Microscopy, Confocal [MESH] |Microscopy, Video [MESH] |Microtubules/metabolism/*physiology [MESH] |Models, Biological [MESH] |Sea Urchins [MESH] |Spermatozoa/metabolism/*physiology [MESH] |Stress, Mechanical [MESH] |Time Factors [MESH]Shape-motion relationships of centering microtubule asters (epmc).pdf DeepDyve Pubget Overpricing