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2018 ; 8
(1
): 10895
Nephropedia Template TP
Sladkova M
; Alawadhi R
; Jaragh Alhaddad R
; Esmael A
; Alansari S
; Saad M
; Mulla Yousef J
; Alqaoud L
; de Peppo GM
Sci Rep
2018[Jul]; 8
(1
): 10895
PMID30022102
show ga
Segmental bone defects caused by trauma and disease represent a major clinical
problem worldwide. Current treatment options are limited and often associated
with poor outcomes and severe complications. Bone engineering is a promising
alternative solution, but a number of technical challenges must be addressed to
allow for effective and reproducible construction of segmental grafts that meet
the size and geometrical requirements needed for individual patients and routine
clinical applications. It is important to devise engineering strategies and
standard operating procedures that make it possible to scale up the size of
bone-engineered grafts, minimize process and product variability, and facilitate
technology transfer and implementation. To address these issues, we have combined
traditional and modular tissue engineering approaches in a strategy referred to
as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a
digital reconstruction of a rabbit femoral defect was partitioned transversally
to the longitudinal axis into segments (modules) with discoidal geometry and
defined thickness to enable protocol standardization and effective tissue
formation in vitro. Bone grafts corresponding to each segment were then
engineered using biomimetic scaffolds seeded with human induced pluripotent stem
cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor
with universal design. The SATE strategy enables the effective and reproducible
engineering of segmental bone grafts for personalized skeletal reconstruction,
and will facilitate technology transfer and implementation of a tissue
engineering approach to segmental bone defect therapy.
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