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2013 ; 15
(33
): 13705-12
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Seeing the chemistry in biology with neutron crystallography
#MMPMID23852376
Langan P
; Chen JC
Phys Chem Chem Phys
2013[Sep]; 15
(33
): 13705-12
PMID23852376
show ga
New developments in macromolecular neutron crystallography have led to an
increasing number of structures published over the last decade. Hydrogen atoms,
normally invisible in most X-ray crystal structures, become visible with
neutrons. Using X-rays allows one to see structure, while neutrons allow one to
reveal the chemistry inherent in these macromolecular structures. A number of
surprising and sometimes controversial results have emerged; because it is
difficult to see or predict hydrogen atoms in X-ray structures, when they are
seen by neutrons they can be in unexpected locations with important chemical and
biological consequences. Here we describe examples of chemistry seen with
neutrons for the first time in biological macromolecules over the past few years.