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10.1002/anie.201411692 http://scihub22266oqcxt.onion/10.1002/anie.201411692 C4471591!4471591 !25783034     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25783034 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Angew+Chem+Int+Ed+Engl 2015 ; 54 (19 ): 5784-8 Nephropedia Template TP {{tp|p=25783034 |t=2015. Secondary-ion mass spectrometry of genetically encoded targets |pdf=|usr=}}{{25783034 }} gab.com Text Secondary-ion mass spectrometry of genetically encoded targets JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25783034 #FOAvip Secondary ion mass spectrometry (SIMS) is generally used in imaging the isotopic composition of various materials. It is becoming increasingly popular in biology, especially for investigations of cellular metabolism. However, individual proteins are difficult to identify in SIMS, which limits the ability of this technology to study individual compartments or protein complexes. We present a method for specific protein isotopic and fluorescence labeling (SPILL), based on a novel click reaction with isotopic probes. Using this method, we added (19) F-enriched labels to different proteins, and visualized them by NanoSIMS and fluorescence microscopy. The (19) F signal allowed the precise visualization of the protein of interest, with minimal background, and enabled correlative studies of protein distribution and cellular metabolism or composition. SPILL can be applied to biological systems suitable for click chemistry, which include most cell-culture systems, as well as small model organisms. Twit Text FOAVip Secondary-ion mass spectrometry of genetically encoded targets ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25783034 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25783034 #FOAvip English Wikipedia <ref>{{Cite pmid|25783034 }}</ref> Secondary-ion mass spectrometry of genetically encoded targets #MMPMID25783034 Vreja IC ; Kabatas S ; Saka SK ; Kröhnert K ; Höschen C ; Opazo F ; Diederichsen U ; Rizzoli SO Angew Chem Int Ed Engl 2015[May]; 54 (19 ): 5784-8 PMID25783034 show ga Secondary ion mass spectrometry (SIMS) is generally used in imaging the isotopic composition of various materials. It is becoming increasingly popular in biology, especially for investigations of cellular metabolism. However, individual proteins are difficult to identify in SIMS, which limits the ability of this technology to study individual compartments or protein complexes. We present a method for specific protein isotopic and fluorescence labeling (SPILL), based on a novel click reaction with isotopic probes. Using this method, we added (19) F-enriched labels to different proteins, and visualized them by NanoSIMS and fluorescence microscopy. The (19) F signal allowed the precise visualization of the protein of interest, with minimal background, and enabled correlative studies of protein distribution and cellular metabolism or composition. SPILL can be applied to biological systems suitable for click chemistry, which include most cell-culture systems, as well as small model organisms. |*Nanotechnology [MESH] |*Spectrometry, Mass, Secondary Ion [MESH] |Animals [MESH] |Cell Line [MESH] |Click Chemistry [MESH] |Cricetinae [MESH] |Fluorescent Dyes/chemistry [MESH] |Fluorine Radioisotopes [MESH] |Microscopy, Fluorescence [MESH] |Molecular Structure [MESH]Secondary-ion mass spectrometry of genetically encoded targets (epmc).pdf DeepDyve Pubget Overpricing