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2016 ; 133
(ä): 55-68
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Second harmonic generation microscopy in zebrafish
#MMPMID27263408
LeBert DC
; Squirrell JM
; Huttenlocher A
; Eliceiri KW
Methods Cell Biol
2016[]; 133
(ä): 55-68
PMID27263408
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Modern optical imaging has progressed rapidly with the ability to noninvasively
image cellular and subcellular phenomena with high spatial and temporal
resolution. In particular, emerging techniques such as second harmonic generation
(SHG) microscopy can allow for the monitoring of intrinsic contrast, such as that
from collagen, in live and fixed samples. When coupled with multiphoton
fluorescence microscopy, SHG can be used to image interactions between cells and
the surrounding extracellular environment. There is recent interest in using
these approaches to study inflammation and wound healing in zebrafish, an
important model for studying these processes. In this chapter we present the
practical aspects of using second harmonic generation to image interactions
between leukocytes and collagen during wound healing in zebrafish.