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10.1038/ncomms8127 http://scihub22266oqcxt.onion/10.1038/ncomms8127 C4479004!4479004 !25980788     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25980788 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2015 ; 6 (ä): 7127 Nephropedia Template TP {{tp|p=25980788 |t=2015. STED nanoscopy with fluorescent quantum dots |pdf=|usr=}}{{25980788 }} gab.com Text STED nanoscopy with fluorescent quantum dots JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=25980788 #FOAvip The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discovery that STED nanoscopy of several red-emitting commercially available quantum dots is in fact successfully realized by the increasingly popular 775 nm STED laser light. A resolution of presently ? 50 nm is demonstrated for single quantum dots, and sub-diffraction resolution is further shown for imaging of quantum-dot-labelled vimentin filaments in fibroblasts. The high quantum-dot photostability enables repeated STED recordings with >1,000 frames. In addition, we have evidence that the tendency of quantum-dot labels to blink is largely suppressed by combined action of excitation and STED beams. Quantum-dot STED significantly expands the realm of application of STED nanoscopy, and, given the high stability of these probes, holds promise for extended time-lapse imaging. Twit Text FOAVip STED nanoscopy with fluorescent quantum dots ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=25980788 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25980788 #FOAvip English Wikipedia <ref>{{Cite pmid|25980788 }}</ref> STED nanoscopy with fluorescent quantum dots #MMPMID25980788 Hanne J ; Falk HJ ; Görlitz F ; Hoyer P ; Engelhardt J ; Sahl SJ ; Hell SW Nat Commun 2015[May]; 6 (ä): 7127 PMID25980788 show ga The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discovery that STED nanoscopy of several red-emitting commercially available quantum dots is in fact successfully realized by the increasingly popular 775 nm STED laser light. A resolution of presently ? 50 nm is demonstrated for single quantum dots, and sub-diffraction resolution is further shown for imaging of quantum-dot-labelled vimentin filaments in fibroblasts. The high quantum-dot photostability enables repeated STED recordings with >1,000 frames. In addition, we have evidence that the tendency of quantum-dot labels to blink is largely suppressed by combined action of excitation and STED beams. Quantum-dot STED significantly expands the realm of application of STED nanoscopy, and, given the high stability of these probes, holds promise for extended time-lapse imaging. äSTED nanoscopy with fluorescent quantum dots (epmc).pdf DeepDyve Pubget Overpricing