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2016 ; 11
(4
): e0153022
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SAMA: A Method for 3D Morphological Analysis
#MMPMID27035711
Paulose T
; Montévil M
; Speroni L
; Cerruti F
; Sonnenschein C
; Soto AM
PLoS One
2016[]; 11
(4
): e0153022
PMID27035711
show ga
Three-dimensional (3D) culture models are critical tools for understanding tissue
morphogenesis. A key requirement for their analysis is the ability to reconstruct
the tissue into computational models that allow quantitative evaluation of the
formed structures. Here, we present Software for Automated Morphological Analysis
(SAMA), a method by which epithelial structures grown in 3D cultures can be
imaged, reconstructed and analyzed with minimum human intervention. SAMA allows
quantitative analysis of key features of epithelial morphogenesis such as ductal
elongation, branching and lumen formation that distinguish different hormonal
treatments. SAMA is a user-friendly set of customized macros operated via FIJI
(http://fiji.sc/Fiji), an open-source image analysis platform in combination with
a set of functions in R (http://www.r-project.org/), an open-source program for
statistical analysis. SAMA enables a rapid, exhaustive and quantitative 3D
analysis of the shape of a population of structures in a 3D image. SAMA is
cross-platform, licensed under the GPLv3 and available at
http://montevil.theobio.org/content/sama.