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10.1016/B978-0-12-801122-5.00015-5 http://scihub22266oqcxt.onion/10.1016/B978-0-12-801122-5.00015-5 C4889436!4889436 !25432756     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25432756 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25432756 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Enzymol 2014 ; 549 (ä): 343-73 Nephropedia Template TP {{tp|p=25432756 |t=2014. Riboswitch structure and dynamics by smFRET microscopy |pdf=|usr=}}{{25432756 }} gab.com Text Riboswitch structure and dynamics by smFRET microscopy JO: Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=25432756 #FOAvip Riboswitches are structured noncoding RNA elements that control the expression of their embedding messenger RNAs by sensing the intracellular concentration of diverse metabolites. As the name suggests, riboswitches are dynamic in nature so that studying their inherent conformational dynamics and ligand-mediated folding is important for understanding their mechanism of action. Single-molecule fluorescence energy transfer (smFRET) microscopy is a powerful and versatile technique for studying the folding pathways and intra- and intermolecular dynamics of biological macromolecules, especially RNA. The ability of smFRET to monitor intramolecular distances and their temporal evolution make it a particularly insightful tool for probing the structure and dynamics of riboswitches. Here, we detail the general steps for using prism-based total internal reflection fluorescence microscopy for smFRET studies of the structure, dynamics, and ligand-binding mechanisms of riboswitches. Twit Text FOAVip Riboswitch structure and dynamics by smFRET microscopy ????Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=25432756 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25432756 #FOAvip English Wikipedia <ref>{{Cite pmid|25432756 }}</ref> Riboswitch structure and dynamics by smFRET microscopy #MMPMID25432756 Suddala KC ; Walter NG Methods Enzymol 2014[]; 549 (ä): 343-73 PMID25432756 show ga Riboswitches are structured noncoding RNA elements that control the expression of their embedding messenger RNAs by sensing the intracellular concentration of diverse metabolites. As the name suggests, riboswitches are dynamic in nature so that studying their inherent conformational dynamics and ligand-mediated folding is important for understanding their mechanism of action. Single-molecule fluorescence energy transfer (smFRET) microscopy is a powerful and versatile technique for studying the folding pathways and intra- and intermolecular dynamics of biological macromolecules, especially RNA. The ability of smFRET to monitor intramolecular distances and their temporal evolution make it a particularly insightful tool for probing the structure and dynamics of riboswitches. Here, we detail the general steps for using prism-based total internal reflection fluorescence microscopy for smFRET studies of the structure, dynamics, and ligand-binding mechanisms of riboswitches. |*Riboswitch [MESH] |Equipment Design [MESH] |Microscopy, Fluorescence/instrumentation/*methods [MESH] |Nucleic Acid Conformation [MESH]Riboswitch structure and dynamics by smFRET microscopy (epmc).pdf DeepDyve Pubget Overpricing