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10.1002/cpch.9 http://scihub22266oqcxt.onion/10.1002/cpch.9 C5613289!5613289 !27622568     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27622568 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Curr+Protoc+Chem+Biol 2016 ; 8 (3 ): 179-196 Nephropedia Template TP {{tp|p=27622568 |t=2016. Reverse Phase Protein Arrays for Compound Profiling |pdf=|usr=}}{{27622568 }} gab.com Text Reverse Phase Protein Arrays for Compound Profiling JO: Curr Protoc Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27622568 #FOAvip Reverse phase protein arrays (RPPAs), also called reverse phase lysate arrays (RPLAs), involve immobilizing cell or tissue lysates, in small spots, onto solid supports which are then probed with primary antibodies specific for proteins or post-translational modifications of interest. RPPA assays are well suited for large-scale, high-throughput measurement of protein and PTM levels in cells and tissues. RPPAs are affordable and highly multiplexable, as a large number of arrays can readily be produced in parallel and then probed separately with distinct primary antibodies. This article describes a procedure for treating cells and preparing cell lysates, as well as a procedure for generating RPPAs using these lysates. A method for probing, imaging, and analyzing RPPAs is also described. These procedures are readily adaptable to a wide range of studies of cell signaling in response to drugs and other perturbations. © 2016 by John Wiley & Sons, Inc. Twit Text FOAVip Reverse Phase Protein Arrays for Compound Profiling ????Curr Protoc Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27622568 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27622568 #FOAvip English Wikipedia <ref>{{Cite pmid|27622568 }}</ref> Reverse Phase Protein Arrays for Compound Profiling #MMPMID27622568 Moerke N ; Fallahi-Sichani M Curr Protoc Chem Biol 2016[Sep]; 8 (3 ): 179-196 PMID27622568 show ga Reverse phase protein arrays (RPPAs), also called reverse phase lysate arrays (RPLAs), involve immobilizing cell or tissue lysates, in small spots, onto solid supports which are then probed with primary antibodies specific for proteins or post-translational modifications of interest. RPPA assays are well suited for large-scale, high-throughput measurement of protein and PTM levels in cells and tissues. RPPAs are affordable and highly multiplexable, as a large number of arrays can readily be produced in parallel and then probed separately with distinct primary antibodies. This article describes a procedure for treating cells and preparing cell lysates, as well as a procedure for generating RPPAs using these lysates. A method for probing, imaging, and analyzing RPPAs is also described. These procedures are readily adaptable to a wide range of studies of cell signaling in response to drugs and other perturbations. © 2016 by John Wiley & Sons, Inc. |High-Throughput Screening Assays/methods [MESH] |Humans [MESH] |Optical Imaging/methods [MESH] |Protein Array Analysis/*methods [MESH]Reverse Phase Protein Arrays for Compound Profiling (epmc).pdf DeepDyve Pubget Overpricing