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2015 ; 5
(ä): 13591
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Retinal synaptic regeneration via microfluidic guiding channels
#MMPMID26314276
Su PJ
; Liu Z
; Zhang K
; Han X
; Saito Y
; Xia X
; Yokoi K
; Shen H
; Qin L
Sci Rep
2015[Aug]; 5
(ä): 13591
PMID26314276
show ga
In vitro culture of dissociated retinal neurons is an important model for
investigating retinal synaptic regeneration (RSR) and exploring potentials in
artificial retina. Here, retinal precursor cells were cultured in a microfluidic
chip with multiple arrays of microchannels in order to reconstruct the retinal
neuronal synapse. The cultured retinal cells were physically connected through
microchannels. Activation of electric signal transduction by the cells through
the microchannels was demonstrated by administration of glycinergic factors. In
addition, an image-based analytical method was used to quantify the synaptic
connections and to assess the kinetics of synaptic regeneration. The rate of RSR
decreased significantly below 100??M of inhibitor glycine and then approached to
a relatively constant level at higher concentrations. Furthermore, RSR was
enhanced by chemical stimulation with potassium chloride. Collectively, the
microfluidic synaptic regeneration chip provides a novel tool for high-throughput
investigation of RSR at the cellular level and may be useful in quality control
of retinal precursor cell transplantation.
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