Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24970197
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Biomolecules
2013 ; 3
(4
): 923-42
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Research applications of proteolytic enzymes in molecular biology
#MMPMID24970197
Mótyán JA
; Tóth F
; T?zsér J
Biomolecules
2013[Nov]; 3
(4
): 923-42
PMID24970197
show ga
Proteolytic enzymes (also termed peptidases, proteases and proteinases) are
capable of hydrolyzing peptide bonds in proteins. They can be found in all living
organisms, from viruses to animals and humans. Proteolytic enzymes have great
medical and pharmaceutical importance due to their key role in biological
processes and in the life-cycle of many pathogens. Proteases are extensively
applied enzymes in several sectors of industry and biotechnology, furthermore,
numerous research applications require their use, including production of Klenow
fragments, peptide synthesis, digestion of unwanted proteins during nucleic acid
purification, cell culturing and tissue dissociation, preparation of recombinant
antibody fragments for research, diagnostics and therapy, exploration of the
structure-function relationships by structural studies, removal of affinity tags
from fusion proteins in recombinant protein techniques, peptide sequencing and
proteolytic digestion of proteins in proteomics. The aim of this paper is to
review the molecular biological aspects of proteolytic enzymes and summarize
their applications in the life sciences.