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10.1016/j.urology.2016.02.003 http://scihub22266oqcxt.onion/10.1016/j.urology.2016.02.003 C4891186!4891186 !26892647     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26892647 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Urology 2016 ; 92 (ä): 100-5 Nephropedia Template TP {{tp|p=26892647 |t=2016. Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidence |pdf=|usr=}}{{26892647 }} gab.com Text Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidence JO: Urology http://www.kidney.de/pget.php?&tw=1&pmid=26892647 #FOAvip OBJECTIVE: To investigate whether inflammation, oxidation, and tubular injury are present in children with stones (RS) compared with healthy controls (HC) by measuring urinary proteins involved in these processes. METHODS: Quantitative proteomic comparison of pooled urine from RS (N?=?30, 24 females, mean age 12.95?±?4.03 years) versus age- and gender-matched HC (N?=?30), using liquid chromatography-mass spectrometry. Relative protein abundance was estimated using spectral counting. Proteins of interest were selected using the following criteria: (1) ?5 spectral counts; (2) ?2-fold difference in spectral counts; and (3) P-value ?.05 for Fisher's exact test. RESULTS: Of the 1813 proteins identified, 230 met the above criteria, with 163 proteins upregulated in the RS group and 67 upregulated in HC. Functional analysis revealed 19 inflammatory proteins, 5 proteins involved in oxidative stress, and 5 involved in tubular injury. Of those proteins, NADPH-oxidase, a major source of reactive oxygen species, was only found in the RS group, whereas glutathione S-transferase A2, an important antioxidant enzyme, was more abundant in controls. ELISA analysis confirmed statistically significant differences in the urinary excretion of retinol-binding protein 4, a marker of proximal tubular dysfunction, between stone patients with hypercalciuria and controls. CONCLUSION: We provide proteomic evidence of oxidative stress, inflammation, and tubular injury in children with renal stones. We speculate that inflammation and changes in the oxidant-antioxidant balance may cause tubular damage in these patients. Targeting these proteins may have therapeutic benefits. Twit Text FOAVip Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidence ????Urology http://www.kidney.de/pget.php?&tw=1&pmid=26892647 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26892647 #FOAvip English Wikipedia <ref>{{Cite pmid|26892647 }}</ref> Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidence #MMPMID26892647 Kovacevic L ; Lu H ; Caruso JA ; Lakshmanan Y Urology 2016[Jun]; 92 (ä): 100-5 PMID26892647 show ga OBJECTIVE: To investigate whether inflammation, oxidation, and tubular injury are present in children with stones (RS) compared with healthy controls (HC) by measuring urinary proteins involved in these processes. METHODS: Quantitative proteomic comparison of pooled urine from RS (N?=?30, 24 females, mean age 12.95?±?4.03 years) versus age- and gender-matched HC (N?=?30), using liquid chromatography-mass spectrometry. Relative protein abundance was estimated using spectral counting. Proteins of interest were selected using the following criteria: (1) ?5 spectral counts; (2) ?2-fold difference in spectral counts; and (3) P-value ?.05 for Fisher's exact test. RESULTS: Of the 1813 proteins identified, 230 met the above criteria, with 163 proteins upregulated in the RS group and 67 upregulated in HC. Functional analysis revealed 19 inflammatory proteins, 5 proteins involved in oxidative stress, and 5 involved in tubular injury. Of those proteins, NADPH-oxidase, a major source of reactive oxygen species, was only found in the RS group, whereas glutathione S-transferase A2, an important antioxidant enzyme, was more abundant in controls. ELISA analysis confirmed statistically significant differences in the urinary excretion of retinol-binding protein 4, a marker of proximal tubular dysfunction, between stone patients with hypercalciuria and controls. CONCLUSION: We provide proteomic evidence of oxidative stress, inflammation, and tubular injury in children with renal stones. We speculate that inflammation and changes in the oxidant-antioxidant balance may cause tubular damage in these patients. Targeting these proteins may have therapeutic benefits. |*Proteomics [MESH] |Adolescent [MESH] |Child [MESH] |Child, Preschool [MESH] |Female [MESH] |Humans [MESH] |Kidney Calculi/complications/metabolism/*physiopathology/*urine [MESH] |Kidney Tubules/*physiopathology [MESH] |Male [MESH] |Oxidative Stress [MESH] |Prospective Studies [MESH]Renal Tubular Dysfunction in Pediatric Urolithiasis: Proteomic Evidenc(epmc).pdf DeepDyve Pubget Overpricing