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2015 ; 8
(12
): 8590-8607
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Recent Progress in Lectin-Based Biosensors
#MMPMID28793731
Wang B
; Anzai JI
Materials (Basel)
2015[Dec]; 8
(12
): 8590-8607
PMID28793731
show ga
This article reviews recent progress in the development of lectin-based
biosensors used for the determination of glucose, pathogenic bacteria and toxins,
cancer cells, and lectins. Lectin proteins have been widely used for the
construction of optical and electrochemical biosensors by exploiting the specific
binding affinity to carbohydrates. Among lectin proteins, concanavalin A (Con A)
is most frequently used for this purpose as glucose- and mannose-selective
lectin. Con A is useful for immobilizing enzymes including glucose oxidase (GOx)
and horseradish peroxidase (HRP) on the surface of a solid support to construct
glucose and hydrogen peroxide sensors, because these enzymes are covered with
intrinsic hydrocarbon chains. Con A-modified electrodes can be used as biosensors
sensitive to glucose, cancer cells, and pathogenic bacteria covered with
hydrocarbon chains. The target substrates are selectively adsorbed to the surface
of Con A-modified electrodes through strong affinity of Con A to hydrocarbon
chains. A recent topic in the development of lectin-based biosensors is a
successful use of nanomaterials, such as metal nanoparticles and carbon
nanotubes, for amplifying output signals of the sensors. In addition,
lectin-based biosensors are useful for studying glycan expression on living
cells.
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