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10.1038/ncomms11077 http://scihub22266oqcxt.onion/10.1038/ncomms11077 C4821995!4821995 !27032979     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27032979 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27032979 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2016 ; 7 (ä): 11077 Nephropedia Template TP {{tp|p=27032979 |t=2016. Real-time high dynamic range laser scanning microscopy |pdf=|usr=}}{{27032979 }} gab.com Text Real-time high dynamic range laser scanning microscopy JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27032979 #FOAvip In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem of selective data display, we developed a new method that extends the imaging dynamic range in optical microscopy and improves the signal-to-noise ratio. Here we demonstrate how real-time and sequential high dynamic range microscopy facilitates automated three-dimensional neural segmentation. We address reconstruction and segmentation performance on samples with different size, anatomy and complexity. Finally, in vivo real-time high dynamic range imaging is also demonstrated, making the technique particularly relevant for longitudinal imaging in the presence of physiological motion and/or for quantification of in vivo fast tracer kinetics during functional imaging. Twit Text FOAVip Real-time high dynamic range laser scanning microscopy ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27032979 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27032979 #FOAvip English Wikipedia <ref>{{Cite pmid|27032979 }}</ref> Real-time high dynamic range laser scanning microscopy #MMPMID27032979 Vinegoni C ; Leon Swisher C ; Fumene Feruglio P ; Giedt RJ ; Rousso DL ; Stapleton S ; Weissleder R Nat Commun 2016[Apr]; 7 (ä): 11077 PMID27032979 show ga In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem of selective data display, we developed a new method that extends the imaging dynamic range in optical microscopy and improves the signal-to-noise ratio. Here we demonstrate how real-time and sequential high dynamic range microscopy facilitates automated three-dimensional neural segmentation. We address reconstruction and segmentation performance on samples with different size, anatomy and complexity. Finally, in vivo real-time high dynamic range imaging is also demonstrated, making the technique particularly relevant for longitudinal imaging in the presence of physiological motion and/or for quantification of in vivo fast tracer kinetics during functional imaging. |Algorithms [MESH] |Animals [MESH] |Brain/pathology [MESH] |Mice [MESH] |Microscopy, Confocal/*methods [MESH] |Microscopy, Fluorescence, Multiphoton [MESH]Real-time high dynamic range laser scanning microscopy (epmc).pdf DeepDyve Pubget Overpricing