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2014 ; 19
(9
): 90505
Nephropedia Template TP
J Biomed Opt
2014[Sep]; 19
(9
): 90505
PMID25250826
show ga
We introduce a technique that limits absorption effects in fluorescence imaging
and does not require extensive imaging processing, thus allowing for video rate
imaging. The absorption minimization is achieved using spatial frequency domain
imaging at a single high spatial frequency with standard three-phase
demodulation. At a spatial frequency f ¼ 0.5 mm?1, we demonstrated in both
in-vitro phantoms and ex-vivo tissue that the absorption can be significantly
reduced. In the real-time implementation, we achieved a video rate of 19
frames?s. This technique has potential in cancer visualization and tumor margin
detection.