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2016 ; 7
(11
): 4375-4387
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Rapid mesoscale multiphoton microscopy of human skin
#MMPMID27895980
Balu M
; Mikami H
; Hou J
; Potma EO
; Tromberg BJ
Biomed Opt Express
2016[Nov]; 7
(11
): 4375-4387
PMID27895980
show ga
We present a multiphoton microscope designed for mesoscale imaging of human skin.
The system is based on two-photon excited fluorescence and second-harmonic
generation, and images areas of ~0.8x0.8 mm(2) at speeds of 0.8 fps (800x800
pixels; 12 frame averages) for high signal-to-noise ratio, with lateral and axial
resolutions of 0.5µm and 3.3µm, respectively. The main novelty of this instrument
is the design of the scan head, which includes a fast galvanometric scanner,
optimized relay optics, a beam expander and high NA objective lens. Computed
aberrations in focus are below the Marechal criterion of 0.07? rms for
diffraction-limited performance. We demonstrate the practical utility of this
microscope by ex-vivo imaging of wide areas in normal human skin.