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10.1038/s41598-017-13940-w

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suck abstract from ncbi


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pmid29093473
      Sci+Rep 2017 ; 7 (1 ): 14846
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  • Rapid identification of staphylococci by Raman spectroscopy #MMPMID29093473
  • Rebro?ová K ; ?iler M ; Samek O ; R??i?ka F ; Bernatová S ; Holá V ; Je?ek J ; Zemánek P ; Sokolová J ; Petrá? P
  • Sci Rep 2017[Nov]; 7 (1 ): 14846 PMID29093473 show ga
  • Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
  • |Agar [MESH]
  • |Diagnostic Tests, Routine [MESH]
  • |Fluorescence [MESH]
  • |Principal Component Analysis [MESH]
  • |Specimen Handling [MESH]
  • |Spectrum Analysis, Raman/*methods [MESH]
  • |Staphylococcus/*isolation & purification [MESH]


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