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10.1038/s41467-018-04026-w http://scihub22266oqcxt.onion/10.1038/s41467-018-04026-w C5906472!5906472 !29670097     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29670097 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29670097 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2018 ; 9 (1 ): 1530 Nephropedia Template TP {{tp|p=29670097 |t=2018. Random sequences rapidly evolve into de novo promoters |pdf=|usr=}}{{29670097 }} gab.com Text Random sequences rapidly evolve into de novo promoters JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29670097 #FOAvip How new functions arise de novo is a fundamental question in evolution. We studied de novo evolution of promoters in Escherichia coli by replacing the lac promoter with various random sequences of the same size (~100?bp) and evolving the cells in the presence of lactose. We find that ~60% of random sequences can evolve expression comparable to the wild-type with only one mutation, and that ~10% of random sequences can serve as active promoters even without evolution. Such a short mutational distance between random sequences and active promoters may improve the evolvability, yet may also lead to accidental promoters inside genes that interfere with normal expression. Indeed, our bioinformatic analyses indicate that E. coli was under selection to reduce accidental promoters inside genes by avoiding promoter-like sequences. We suggest that a low threshold for functionality balanced by selection against undesired targets can increase the evolvability by making new beneficial features more accessible. Twit Text FOAVip Random sequences rapidly evolve into de novo promoters ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29670097 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29670097 #FOAvip English Wikipedia <ref>{{Cite pmid|29670097 }}</ref> Random sequences rapidly evolve into de novo promoters #MMPMID29670097 Yona AH ; Alm EJ ; Gore J Nat Commun 2018[Apr]; 9 (1 ): 1530 PMID29670097 show ga How new functions arise de novo is a fundamental question in evolution. We studied de novo evolution of promoters in Escherichia coli by replacing the lac promoter with various random sequences of the same size (~100?bp) and evolving the cells in the presence of lactose. We find that ~60% of random sequences can evolve expression comparable to the wild-type with only one mutation, and that ~10% of random sequences can serve as active promoters even without evolution. Such a short mutational distance between random sequences and active promoters may improve the evolvability, yet may also lead to accidental promoters inside genes that interfere with normal expression. Indeed, our bioinformatic analyses indicate that E. coli was under selection to reduce accidental promoters inside genes by avoiding promoter-like sequences. We suggest that a low threshold for functionality balanced by selection against undesired targets can increase the evolvability by making new beneficial features more accessible. |*Mutation [MESH] |*Promoter Regions, Genetic [MESH] |Amino Acid Motifs [MESH] |Chromosomes, Bacterial/genetics [MESH] |Computational Biology [MESH] |Escherichia coli/*genetics [MESH] |Genome, Bacterial [MESH] |Genomics [MESH] |Glycerol/chemistry [MESH] |Selection, Genetic [MESH]Random sequences rapidly evolve into de novo promoters (epmc).pdf DeepDyve Pubget Overpricing