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10.21769/BioProtoc.1101 http://scihub22266oqcxt.onion/10.21769/BioProtoc.1101 C5415304!5415304 !28480315     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28480315 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28480315 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Bio+Protoc 2014 ; 4 (8 ): ä Nephropedia Template TP {{tp|p=28480315 |t=2014. Radioactive Pulse-Chase Analysis and Immunoprecipitation |pdf=|usr=}}{{28480315 }} gab.com Text Radioactive Pulse-Chase Analysis and Immunoprecipitation JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28480315 #FOAvip Labeling of newly-synthesized polypeptides with radioactive amino acids followed by immunoprecipitation allows quantitative analysis of the fate of a given protein in a time-dependent manner. This biochemical approach is usually used to study a variety of processes, such as protein folding, co-translational modifications, intracellular transport, and even its rate of degradation. Here, I describe step by step a simple technique to both label newly-synthesized influenza A virus (IAV) hemagglutinin (HA) with [(35)S]-methionine and then follow its maturation and transport through the secretory pathway by SDS-PAGE and fluorography (Magadan et al., 2013). Twit Text FOAVip Radioactive Pulse-Chase Analysis and Immunoprecipitation ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28480315 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28480315 #FOAvip English Wikipedia <ref>{{Cite pmid|28480315 }}</ref> Radioactive Pulse-Chase Analysis and Immunoprecipitation #MMPMID28480315 Magadán JG Bio Protoc 2014[Apr]; 4 (8 ): ä PMID28480315 show ga Labeling of newly-synthesized polypeptides with radioactive amino acids followed by immunoprecipitation allows quantitative analysis of the fate of a given protein in a time-dependent manner. This biochemical approach is usually used to study a variety of processes, such as protein folding, co-translational modifications, intracellular transport, and even its rate of degradation. Here, I describe step by step a simple technique to both label newly-synthesized influenza A virus (IAV) hemagglutinin (HA) with [(35)S]-methionine and then follow its maturation and transport through the secretory pathway by SDS-PAGE and fluorography (Magadan et al., 2013). äRadioactive Pulse-Chase Analysis and Immunoprecipitation (epmc).pdf DeepDyve Pubget Overpricing