Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27840389
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Proc+Jpn+Acad+Ser+B+Phys+Biol+Sci
2016 ; 92
(9
): 412-422
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
RNA-based gene circuits for cell regulation
#MMPMID27840389
Karagiannis P
; Fujita Y
; Saito H
Proc Jpn Acad Ser B Phys Biol Sci
2016[]; 92
(9
): 412-422
PMID27840389
show ga
A major goal of synthetic biology is to control cell behavior. RNA-mediated
genetic switches (RNA switches) are devices that serve this purpose, as they can
control gene expressions in response to input signals. In general, RNA switches
consist of two domains: an aptamer domain, which binds to an input molecule, and
an actuator domain, which controls the gene expression. An input binding to the
aptamer can cause the actuator to alter the RNA structure, thus changing access
to translation machinery. The assembly of multiple RNA switches has led to
complex gene circuits for cell therapies, including the selective killing of
pathological cells and purification of cell populations. The inclusion of RNA
binding proteins, such as L7Ae, increases the repertoire and precision of the
circuit. In this short review, we discuss synthetic RNA switches for gene
regulation and their potential therapeutic applications.