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RNA interference is essential for cellular quiescence
#MMPMID27738016
Roche B
; Arcangioli B
; Martienssen RA
Science
2016[Nov]; 354
(6313
): ? PMID27738016
show ga
Quiescent cells play a predominant role in most organisms. Here we identify RNA
interference (RNAi) as a major requirement for quiescence (G(0) phase of the cell
cycle) in Schizosaccharomyces pombe RNAi mutants lose viability at G(0) entry and
are unable to maintain long-term quiescence. We identified suppressors of G(0)
defects in cells lacking Dicer (dcr1?), which mapped to genes involved in
chromosome segregation, RNA polymerase-associated factors, and heterochromatin
formation. We propose a model in which RNAi promotes the release of RNA
polymerase in cycling and quiescent cells: (i) RNA polymerase II release mediates
heterochromatin formation at centromeres, allowing proper chromosome segregation
during mitotic growth and G(0) entry, and (ii) RNA polymerase I release prevents
heterochromatin formation at ribosomal DNA during quiescence maintenance. Our
model may account for the codependency of RNAi and histone H3 lysine 9
methylation throughout eukaryotic evolution.
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