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10.21769/BioProtoc.1358

http://scihub22266oqcxt.onion/10.21769/BioProtoc.1358
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pmid29170745
      Bio+Protoc 2014 ; 4 (24 ): ä
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  • RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans #MMPMID29170745
  • Cecere G ; Grishok A
  • Bio Protoc 2014[Dec]; 4 (24 ): ä PMID29170745 show ga
  • The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA-protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA-protein complexes. Here we describe the RNA-ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope.
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