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10.1038/nmeth.4121 http://scihub22266oqcxt.onion/10.1038/nmeth.4121 C5423094!5423094 !28024160     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28024160 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2017 ; 14 (2 ): 201-207 Nephropedia Template TP {{tp|p=28024160 |t=2017. RESA identifies mRNA-regulatory sequences at high resolution |pdf=|usr=}}{{28024160 }} gab.com Text RESA identifies mRNA-regulatory sequences at high resolution JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA-element selection assay (RESA), a method that selects RNA elements on the basis of their activity in vivo and uses high-throughput sequencing to provide a quantitative measurement of their regulatory functions at near-nucleotide resolution. We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. RESA provides a sensitive and quantitative measure of microRNA activity in vivo and also identifies novel regulatory sequences. To uncover specific sequence requirements within regulatory elements, we developed a bisulfite-mediated nucleotide-conversion strategy for large-scale mutational analysis (RESA-bisulfite). Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP). Twit Text FOAVip RESA identifies mRNA-regulatory sequences at high resolution ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip English Wikipedia <ref>{{Cite pmid|28024160 }}</ref> RESA identifies mRNA-regulatory sequences at high resolution #MMPMID28024160 Yartseva V ; Takacs CM ; Vejnar CE ; Lee MT ; Giraldez AJ Nat Methods 2017[Feb]; 14 (2 ): 201-207 PMID28024160 show ga Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA-element selection assay (RESA), a method that selects RNA elements on the basis of their activity in vivo and uses high-throughput sequencing to provide a quantitative measurement of their regulatory functions at near-nucleotide resolution. We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. RESA provides a sensitive and quantitative measure of microRNA activity in vivo and also identifies novel regulatory sequences. To uncover specific sequence requirements within regulatory elements, we developed a bisulfite-mediated nucleotide-conversion strategy for large-scale mutational analysis (RESA-bisulfite). Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP). |*Genetic Techniques [MESH] |*RNA, Messenger/genetics [MESH] |*Regulatory Sequences, Nucleic Acid [MESH] |3' Untranslated Regions [MESH] |Animals [MESH] |Embryo, Nonmammalian [MESH] |High-Throughput Nucleotide Sequencing/*methods [MESH] |Immunoprecipitation [MESH] |RNA Stability [MESH] |Sulfites [MESH]RESA identifies mRNA-regulatory sequences at high resolution (epmc).pdf DeepDyve Pubget Overpricing