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10.1038/nmeth.3804 http://scihub22266oqcxt.onion/10.1038/nmeth.3804 C4941813!4941813 !27018580     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27018580 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2016 ; 13 (5 ): 439-42 Nephropedia Template TP {{tp|p=27018580 |t=2016. Quantitative super-resolution imaging with qPAINT |pdf=|usr=}}{{27018580 }} gab.com Text Quantitative super-resolution imaging with qPAINT JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target. Twit Text FOAVip Quantitative super-resolution imaging with qPAINT ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip English Wikipedia <ref>{{Cite pmid|27018580 }}</ref> Quantitative super-resolution imaging with qPAINT #MMPMID27018580 Jungmann R ; Avendaņo MS ; Dai M ; Woehrstein JB ; Agasti SS ; Feiger Z ; Rodal A ; Yin P Nat Methods 2016[May]; 13 (5 ): 439-42 PMID27018580 show ga Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target. |Animals [MESH] |DNA/*chemistry/*ultrastructure [MESH] |Fluorescent Dyes/chemistry [MESH] |Humans [MESH] |Image Enhancement/*methods [MESH] |In Situ Hybridization, Fluorescence/*methods [MESH] |Microscopy, Confocal/methods [MESH] |Microscopy, Fluorescence/*methods [MESH] |Molecular Docking Simulation [MESH] |Sequence Analysis, DNA [MESH]Quantitative super-resolution imaging with qPAINT (epmc).pdf DeepDyve Pubget Overpricing