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2014 ; 28
(ä): 112-21
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Quantitative analysis of single-molecule superresolution images
#MMPMID25179006
Coltharp C
; Yang X
; Xiao J
Curr Opin Struct Biol
2014[Oct]; 28
(ä): 112-21
PMID25179006
show ga
This review highlights the quantitative capabilities of single-molecule
localization-based superresolution imaging methods. In addition to revealing fine
structural details, the molecule coordinate lists generated by these methods
provide the critical ability to quantify the number, clustering, and
colocalization of molecules with 10-50 nm resolution. Here we describe typical
workflows and precautions for quantitative analysis of single-molecule
superresolution images. These guidelines include potential pitfalls and essential
control experiments, allowing critical assessment and interpretation of
superresolution images.