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2015 ; 81
(20
): 7098-105
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Quantitative Analysis of Lysobacter Predation
#MMPMID26231654
Seccareccia I
; Kost C
; Nett M
Appl Environ Microbiol
2015[Oct]; 81
(20
): 7098-105
PMID26231654
show ga
Bacteria of the genus Lysobacter are considered to be facultative predators that
use a feeding strategy similar to that of myxobacteria. Experimental data
supporting this assumption, however, are scarce. Therefore, the predatory
activities of three Lysobacter species were tested in the prey spot plate assay
and in the lawn predation assay, which are commonly used to analyze myxobacterial
predation. Surprisingly, only one of the tested Lysobacter species showed
predatory behavior in the two assays. This result suggested that not all
Lysobacter strains are predatory or, alternatively, that the assays were not
appropriate for determining the predatory potential of this bacterial group. To
differentiate between the two scenarios, predation was tested in a CFU-based
bioassay. For this purpose, defined numbers of Lysobacter cells were mixed
together with potential prey bacteria featuring phenotypic markers, such as
distinctive pigmentation or antibiotic resistance. After 24 h, cocultivated cells
were streaked out on agar plates and sizes of bacterial populations were
individually determined by counting the respective colonies. Using the CFU-based
predation assay, we observed that Lysobacter spp. strongly antagonized other
bacteria under nutrient-deficient conditions. Simultaneously, the Lysobacter
population was increasing, which together with the killing of the cocultured
bacteria indicated predation. Variation of the predator/prey ratio revealed that
all three Lysobacter species tested needed to outnumber their prey for efficient
predation, suggesting that they exclusively practiced group predation. In
summary, the CFU-based predation assay not only enabled the quantification of
prey killing and consumption by Lysobacter spp. but also provided insights into
their mode of predation.
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