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2015 ; 3
(6
): 792-796
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Quantification of preeclampsia-related microRNAs in maternal serum
#MMPMID26623017
Li Q
; Long A
; Jiang L
; Cai L
; Xie LI
; Gu J
; Chen X
; Tan L
Biomed Rep
2015[Nov]; 3
(6
): 792-796
PMID26623017
show ga
To identify the specific serum preeclampsia (PE)-related biomarkers, 10 microRNAs
(miRNAs) were selected based on their reported aberrant (4 upregulated and 6
downregulated) expression in PE placenta. A total of 1,035 pregnant patients were
enrolled. Finally, 32 pregnancies with PE and 32 healthy pregnancies were
incorporated in the study. The expression of these 10 miRNAs in the different
trimesters was determined by SYBR-Green reverse transcription-quantitative
polymerase chain reaction. Compared with that in the healthy controls, the
expression levels of miR-152, miR-183 and miR-210 in PE serum were higher in the
second and third trimester, whereas the expression of miR-182 was only higher in
the third trimester. The expression levels of 6 miRNAs (miR-1, miR-328, miR-363,
miR-377, miR-500 and miR-584) that were downregulated in PE placenta showed no
significant differences between pregnancies complicated by PE and healthy
pregnancies throughout the 3 trimesters. Areas under the receiver operating
characteristic [standard error (SE)] during the 20-24th gestational week for
predicting PE were miR-152: 0.94 (SE, 0.026), miR-183: 0.97 (SE, 0.031) and
miR-210: 0.93 (SE, 0.018). In conclusion, the expression levels of serum miR-152,
miR-183 and miR-210 were elevated since the second trimester in pregnancies
complicated with PE, indicating their potentials as serum biomarkers for
forecasting PE.