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2015 ; 15
(6
): 13028-51
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QD-Based FRET Probes at a Glance
#MMPMID26053750
Shamirian A
; Ghai A
; Snee PT
Sensors (Basel)
2015[Jun]; 15
(6
): 13028-51
PMID26053750
show ga
The unique optoelectronic properties of quantum dots (QDs) give them significant
advantages over traditional organic dyes, not only as fluorescent labels for
bioimaging, but also as emissive sensing probes. QD sensors that function via
manipulation of fluorescent resonance energy transfer (FRET) are of special
interest due to the multiple response mechanisms that may be utilized, which in
turn imparts enhanced flexibility in their design. They may also function as
ratiometric, or "color-changing" probes. In this review, we describe the
fundamentals of FRET and provide examples of QD-FRET sensors as grouped by their
response mechanisms such as link cleavage and structural rearrangement. An
overview of early works, recent advances, and various models of QD-FRET sensors
for the measurement of pH and oxygen, as well as the presence of metal ions and
proteins such as enzymes, are also provided.