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2018 ; 38
(3
): ä Nephropedia Template TP
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Pyroptosis in pterygium pathogenesis
#MMPMID29724886
Sun N
; Zhang H
Biosci Rep
2018[May]; 38
(3
): ä PMID29724886
show ga
Pterygium is a common ocular disease characterized by proliferating fibrovascular
tissue. Pyroptosis, a recently discovered programed cell death, is known to be
associated with oxidative stress, one of the main causes of pterygia. Here, we
aimed to study the role of pyroptosis in pterygium pathogenesis. The expression
of nod-like receptor pyrins-3 (NLRP3), caspase-1, IL-18, and IL-1? was analyzed
in 60 human pterygium tissues and 60 human conjunctival epithelium tissues using
real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot
analysis. Human conjunctival epithelial cells (HConECs) and human pterygium
fibroblasts (HPFs) were primary cultured and the level of pyroptosis-associated
factors was detected. Both cells were treated with H(2)O(2), and cell lysis was
detected by lactate dehydrogenase (LDH) release assay, the expression of the
factors by qRT-PCR, Western blot analysis, and immunostaining. The downstream
factors IL-18 and IL-1? were measured after inhibition of caspase-1 to confirm
the caspase-1-dependent pyroptosis. ?-SMA and E-cadherin were detected as
indicators of pyroptosis-induced myofibroblast activation in HPFs. We discovered
that the expression of the factors was significantly increased in pterygium and
that caspase-1-dependent pyroptosis presents in both H(2)O(2)-treated HPFs and
HConECs during which the expression of these factors was significantly elevated
and the elevation of downstream factors IL-18 and IL-1? was restrained after
caspase-1 inhibition. ?-SMA increase and E-cadherin down-regulation were detected
in H(2)O(2)-treated HPFs and the changes were reversed by caspase-1 inhibition.
Pyroptosis displays a role in the pathological process of pterygium formation and
progression. Pyroptosis appears to be an intriguing target to prevent pterygium
pathogenesis.
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