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10.1100/tsw.2002.836

http://scihub22266oqcxt.onion/10.1100/tsw.2002.836
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C6009755!6009755 !12806134
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pmid12806134
      ScientificWorldJournal 2002 ; 2 (ä): 1532-5
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  • Purification of peroxisomes in a self-generated gradient #MMPMID12806134
  • Graham JM
  • ScientificWorldJournal 2002[Jun]; 2 (ä): 1532-5 PMID12806134 show ga
  • In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll there is no contamination from endoplasmic reticulum.
  • |*Peroxisomes [MESH]
  • |Animals [MESH]
  • |Cell Fractionation/*methods [MESH]
  • |Centrifugation, Density Gradient/*methods [MESH]


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