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2002 ; 2
(ä): 1532-5
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Purification of peroxisomes in a self-generated gradient
#MMPMID12806134
Graham JM
ScientificWorldJournal
2002[Jun]; 2
(ä): 1532-5
PMID12806134
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In iodixanol, peroxisomes are the densest organelle in the light mitochondrial
fraction and are therefore easily separated from the other components (lysosomes,
mitochondria, etc.) in a self-generated gradient. Self-generated gradients make
sample handling very easy and are highly reproducible but need to be formed in
either a vertical, near-vertical, or small volume high-performance fixed-angle
rotor. The resolution of the peroxisomes is far superior than that in sucrose
and, unlike in Percoll there is no contamination from endoplasmic reticulum.