10.1002/cpch.1 http://scihub22266oqcxt.onion/10.1002/cpch.1 C4940987!4940987
!27258688
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Curr+Protoc+Chem+Biol
2016 ; 8
(2
): 83-95
Nephropedia Template TP gab.com Text Twit Text FOAVip Twit Text # English Wikipedia
Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation
#MMPMID27258688
Mack S
; Fouz MF
; Dey SK
; Das SR
Curr Protoc Chem Biol
2016[Jun]; 8
(2
): 83-95
PMID27258688
show ga
Particularly for its use in bioconjugations, the copper-catalyzed (or
copper-promoted) azide-alkyne cycloaddition (CuAAC) reaction or 'click
chemistry', has become an essential component of the modern chemical biologist's
toolbox. Click chemistry has been applied to DNA, and more recently, RNA
conjugations, and the protocols presented here can be used for either. The
reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor
co-solvent that serves as a ligand to stabilize the copper. The method also
includes details on the analysis of the reaction product. © 2016 by John Wiley &
Sons, Inc.
Please enable JavaScript to view the comments powered by Disqus. |Alkynes/*chemistry
[MESH] |Azides/*chemistry
[MESH] |Click Chemistry/*methods
[MESH] |DNA/chemistry
[MESH] |Oligonucleotides/*chemistry
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