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10.1016/j.bdq.2015.04.002

http://scihub22266oqcxt.onion/10.1016/j.bdq.2015.04.002
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C4822221!4822221 !27077033
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suck abstract from ncbi


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pmid27077033
      Biomol+Detect+Quantif 2015 ; 4 (ä): 10-6
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  • Proximity assays for sensitive quantification of proteins #MMPMID27077033
  • Greenwood C ; Ruff D ; Kirvell S ; Johnson G ; Dhillon HS ; Bustin SA
  • Biomol Detect Quantif 2015[Jun]; 4 (ä): 10-6 PMID27077033 show ga
  • Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein-protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.
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