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Protrusion fluctuations direct cell motion
#MMPMID24988339
Caballero D
; Voituriez R
; Riveline D
Biophys J
2014[Jul]; 107
(1
): 34-42
PMID24988339
show ga
Many physiological phenomena involve directional cell migration. It is usually
attributed to chemical gradients in vivo. Recently, other cues have been shown to
guide cells in vitro, including stiffness/adhesion gradients or micropatterned
adhesive motifs. However, the cellular mechanism leading to these biased
migrations remains unknown, and, often, even the direction of motion is
unpredictable. In this study, we show the key role of fluctuating protrusions on
ratchet-like structures in driving NIH3T3 cell migration. We identified the
concept of efficient protrusion and an associated direction index. Our analysis
of the protrusion statistics facilitated the quantitative prediction of cell
trajectories in all investigated conditions. We varied the external cues by
changing the adhesive patterns. We also modified the internal cues using drug
treatments, which modified the protrusion activity. Stochasticity affects the
short- and long-term steps. We developed a theoretical model showing that an
asymmetry in the protrusion fluctuations is sufficient for predicting all
measures associated with the long-term motion, which can be described as a biased
persistent random walk.
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