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10.1371/journal.pone.0175293 http://scihub22266oqcxt.onion/10.1371/journal.pone.0175293 C5383329!5383329 !28384246     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28384246 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28384246 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2017 ; 12 (4 ): e0175293 Nephropedia Template TP {{tp|p=28384246 |t=2017. Proton and non-proton activation of ASIC channels |pdf=|usr=}}{{28384246 }} gab.com Text Proton and non-proton activation of ASIC channels JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28384246 #FOAvip The Acid-Sensing Ion Channels (ASIC) exhibit a fast desensitizing current when activated by pH values below 7.0. By contrast, non-proton ligands are able to trigger sustained ASIC currents at physiological pHs. To analyze the functional basis of the ASIC desensitizing and sustained currents, we have used ASIC1a and ASIC2a mutants with a cysteine in the pore vestibule for covalent binding of different sulfhydryl reagents. We found that ASIC1a and ASIC2a exhibit two distinct currents, a proton-induced desensitizing current and a sustained current triggered by sulfhydryl reagents. These currents differ in their pH dependency, their sensitivity to the sulfhydryl reagents, their ionic selectivity and their relative magnitude. We propose a model for ASIC1 and ASIC2 activity where the channels can function in two distinct modes, a desensitizing mode and a sustained mode depending on the activating ligands. The pore vestibule of the channel represents a functional site for binding non-proton ligands to activate ASIC1 and ASIC2 at neutral pH and to prevent channel desensitization. Twit Text FOAVip Proton and non-proton activation of ASIC channels ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28384246 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28384246 #FOAvip English Wikipedia <ref>{{Cite pmid|28384246 }}</ref> Proton and non-proton activation of ASIC channels #MMPMID28384246 Gautschi I ; van Bemmelen MX ; Schild L PLoS One 2017[]; 12 (4 ): e0175293 PMID28384246 show ga The Acid-Sensing Ion Channels (ASIC) exhibit a fast desensitizing current when activated by pH values below 7.0. By contrast, non-proton ligands are able to trigger sustained ASIC currents at physiological pHs. To analyze the functional basis of the ASIC desensitizing and sustained currents, we have used ASIC1a and ASIC2a mutants with a cysteine in the pore vestibule for covalent binding of different sulfhydryl reagents. We found that ASIC1a and ASIC2a exhibit two distinct currents, a proton-induced desensitizing current and a sustained current triggered by sulfhydryl reagents. These currents differ in their pH dependency, their sensitivity to the sulfhydryl reagents, their ionic selectivity and their relative magnitude. We propose a model for ASIC1 and ASIC2 activity where the channels can function in two distinct modes, a desensitizing mode and a sustained mode depending on the activating ligands. The pore vestibule of the channel represents a functional site for binding non-proton ligands to activate ASIC1 and ASIC2 at neutral pH and to prevent channel desensitization. |*Protons [MESH] |Acid Sensing Ion Channels/*metabolism [MESH] |Hydrogen-Ion Concentration [MESH] |Indicators and Reagents/chemistry [MESH]Proton and non-proton activation of ASIC channels (epmc).pdf DeepDyve Pubget Overpricing